Hotspot

trnG (UCC)-trnR (UCU)

LSC · 9643-9984 · 342 bp

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Pi 0.0269

MarkerSeek score 69.00

Primer available yes

Label trnG (UCC)-trnR (UCU)

Feature Metadata

label_name: trnG (UCC)-trnR (UCU)
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 63
pi: 0.0269
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG (UCC)-trnR (UCU)_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	422-486 bp	1.0	75.3
trnG (UCC)-trnR (UCU)_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	425-489 bp	1.0	75.1
trnG (UCC)-trnR (UCU)_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	428-492 bp	1.0	74.7
trnG (UCC)-trnR (UCU)_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	431-495 bp	1.0	74.5
trnG (UCC)-trnR (UCU)_p5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.7	56.9	426-490 bp	1.0	72.9