Hotspot

trnS (GCU)-trnG (UCC)

LSC · 8213-8875 · 663 bp

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Pi 0.00766

MarkerSeek score 67.10

Primer available yes

Label trnS (GCU)-trnG (UCC)

Feature Metadata

label_name: trnS (GCU)-trnG (UCC)
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 47
pi: 0.00766
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS (GCU)-trnG (UCC)_p1	ATTAGCAATCCGCCGCTTTA	TGAATTCAACCGAAAGACCCT	45.0	42.9	58.0	57.5	619-793 bp	0.977778	76.1
trnS (GCU)-trnG (UCC)_p2	AGGAAACGGAAAGAGAGGGA	TGAATTCAACCGAAAGACCCT	50.0	42.9	58.0	57.5	673-847 bp	0.977778	75.8
trnS (GCU)-trnG (UCC)_p3	ACGGAAAGAGAGGGATTCGA	TGAATTCAACCGAAAGACCCT	50.0	42.9	58.2	57.5	668-842 bp	0.977778	74.8
trnS (GCU)-trnG (UCC)_p4	AGGAAACGGAAAGAGAGGGA	GCACGAATCACACTTTTACCA	50.0	42.9	58.0	57.1	630-804 bp	1.0	73.5
trnS (GCU)-trnG (UCC)_p5	GCTTTAGTCCACTCAGCCAT	TGAATTCAACCGAAAGACCCT	50.0	42.9	57.6	57.5	605-779 bp	0.977778	73.2