Hotspot

rps16-trnQ (UUG)

LSC · 5479-6886 · 1408 bp

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Pi 0.0125

MarkerSeek score 71.00

Primer available yes

Label rps16-trnQ (UUG)

Feature Metadata

label_name: rps16-trnQ (UUG)
feature_type: igs
region: LSC
length_bp: 1408
variable_sites: 153
pi: 0.0125
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ (UUG)_p1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.9	58.0	1455-1616 bp	1.0	91.2
rps16-trnQ (UUG)_p2	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1438-1599 bp	1.0	90.7
rps16-trnQ (UUG)_p3	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1456-1617 bp	1.0	90.1
rps16-trnQ (UUG)_p4	GGATCGTGTCCTTCAAGTCG	GAAATTGAAATGGGGCGTGG	55.0	50.0	58.4	58.0	1452-1613 bp	1.0	88.9
rps16-trnQ (UUG)_p5	CGGATCGTGTCCTTCAAGTC	GAAATTGAAATGGGGCGTGG	55.0	50.0	58.4	58.0	1453-1614 bp	1.0	88.8