Hotspot

trnN-GUU-trnR-ACG

SSC · 88864-89392 · 529 bp

Back to Zygophyllum

Pi 0.039

MarkerSeek score 78.30

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 529
variable_sites: 41
pi: 0.039
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	521-634 bp	1.0	86.6
trnN-GUU-trnR-ACG_p2	TTCGAATCCTACCTGGGGAG	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.2	57.8	511-624 bp	1.0	86.3
trnN-GUU-trnR-ACG_p3	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	525-638 bp	1.0	85.7
trnN-GUU-trnR-ACG_p4	CTCAGTAGCTCAGCGGTAGA	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.3	57.8	561-674 bp	1.0	84.9
trnN-GUU-trnR-ACG_p5	TCAGTAGCTCAGCGGTAGAG	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.3	57.8	560-673 bp	1.0	84.9