Hotspot

psaC-rps15

SSC · 86220-87181 · 962 bp

Back to Zygophyllum

Pi 0.0192

MarkerSeek score 74.90

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 962
variable_sites: 38
pi: 0.0192
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	ACATCCGTCCCAAGGTATCA	AATTTTGGGAAAACGGCAAC	50.0	40.0	58.1	55.9	1173-1323 bp	1.0	66.3
psaC-rps15_p2	CAAGCTCGGACACATTGAGT	AATTTTGGGAAAACGGCAAC	50.0	40.0	58.2	55.9	1133-1283 bp	1.0	65.9
psaC-rps15_p3	AATACATCTGTGGGGCAAGC	AATTTTGGGAAAACGGCAAC	50.0	40.0	58.2	55.9	1148-1298 bp	1.0	65.6
psaC-rps15_p4	CGGACACATTGAGTACACCC	AATTTTGGGAAAACGGCAAC	55.0	40.0	58.3	55.9	1127-1277 bp	1.0	65.3
psaC-rps15_p5	ACATCCGTCCCAAGGTATCA	AAATTTTGGGAAAACGGCAAC	50.0	38.1	58.1	56.7	1174-1324 bp	1.0	64.5