Hotspot

rpl32-trnL-UAG

SSC · 83803-84302 · 500 bp

Back to Zygophyllum

Pi 0.031

MarkerSeek score 71.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 500
variable_sites: 29
pi: 0.031
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	592-647 bp	1.0	85.4
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.8	57.8	585-640 bp	1.0	84.9
rpl32-trnL-UAG_p3	GGGCAGCATTGAAAGCTTTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.8	57.8	591-646 bp	1.0	84.8
rpl32-trnL-UAG_p4	GGGCAGCATTGAAAGCTTTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.8	57.8	590-645 bp	1.0	84.8
rpl32-trnL-UAG_p5	GGGCAGCATTGAAAGCTTTT	GAGCAGCGTGTCTACCAATT	45.0	50.0	57.8	58.3	586-641 bp	1.0	84.2