Hotspot

trnL-CAA-rpl32

IRb · 82993-83646 · 654 bp

Back to Zygophyllum

Pi 0.0504

MarkerSeek score 85.50

Primer available yes

Label trnL-CAA-rpl32

Feature Metadata

label_name: trnL-CAA-rpl32
feature_type: igs
region: IRb
length_bp: 654
variable_sites: 61
pi: 0.0504
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-rpl32_p1	GTCTCGCGTGTCTACCATTT	TACGCTTCTTTGGAACTGCC	50.0	50.0	58.0	58.5	564-818 bp	1.0	87.0
trnL-CAA-rpl32_p2	CTCGCGTGTCTACCATTTCA	TACGCTTCTTTGGAACTGCC	50.0	50.0	58.0	58.5	562-816 bp	1.0	87.0
trnL-CAA-rpl32_p3	TACCATTTCACCACCAAGGC	TACGCTTCTTTGGAACTGCC	50.0	50.0	58.1	58.5	552-806 bp	1.0	86.5
trnL-CAA-rpl32_p4	GTCTCGCGTGTCTACCATTT	GTACGCTTCTTTGGAACTGC	50.0	50.0	58.0	57.4	565-819 bp	1.0	86.0
trnL-CAA-rpl32_p5	CTCGCGTGTCTACCATTTCA	GTACGCTTCTTTGGAACTGC	50.0	50.0	58.0	57.4	563-817 bp	1.0	86.0