Hotspot

psaJ-rpl33

LSC · 62777-63266 · 490 bp

Back to Zygophyllum

Pi 0.0357

MarkerSeek score 72.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 33
pi: 0.0357
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCTCTGTGGCACCGGTATTA	TACCCGGACATCTTTCCCTT	50.0	50.0	58.1	58.0	617-640 bp	1.0	84.9
psaJ-rpl33_p2	TCTCTGTGGCACCGGTATTA	TTACCCGGACATCTTTCCCT	50.0	50.0	58.1	58.0	618-641 bp	1.0	84.9
psaJ-rpl33_p3	TGGTTCGGTTCTTTAGCAGG	TACCCGGACATCTTTCCCTT	50.0	50.0	57.8	58.0	588-611 bp	1.0	84.9
psaJ-rpl33_p4	CATATCTCTCTGTGGCACCG	TACCCGGACATCTTTCCCTT	55.0	50.0	57.9	58.0	623-646 bp	1.0	84.8
psaJ-rpl33_p5	CATATCTCTCTGTGGCACCG	TTACCCGGACATCTTTCCCT	55.0	50.0	57.9	58.0	624-647 bp	1.0	84.8