Hotspot

atpH-atpI

LSC · 12818-13334 · 517 bp

Back to Zygophyllum

Pi 0.0128

MarkerSeek score 71.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 517
variable_sites: 15
pi: 0.0128
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	CCTGTCATGTTCCTTGGGTT	45.0	50.0	58.1	57.7	610-657 bp	1.0	82.7
atpH-atpI_p2	AACAGAAGCGGCAGAAATCA	CTTTGCAACTTTAGCTGCGG	45.0	50.0	58.1	58.3	563-610 bp	1.0	82.7
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	CAACTTTAGCTGCGGCCTAT	45.0	50.0	58.1	58.3	558-605 bp	1.0	82.5
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	CCTGTCATGTTCCTTGGGTT	50.0	50.0	57.8	57.7	620-667 bp	1.0	82.1
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	CTTTGCAACTTTAGCTGCGG	50.0	50.0	57.8	58.3	573-620 bp	1.0	82.1