Hotspot

trnS-GCU-trnS-CGA

LSC · 6857-7824 · 968 bp

Back to Zygophyllum

Pi 0.0465

MarkerSeek score 82.70

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 968
variable_sites: 89
pi: 0.0465
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	GCTTTAGTCCACTCAGCCAT	AGGGTTTTCATCAAACGAATCAC	50.0	39.1	57.6	57.9	1042-1178 bp	1.0	61.3
trnS-GCU-trnS-CGA_p2	GCTTTAGTCCACTCAGCCAT	GGGTTTTCATCAAACGAATCACA	50.0	39.1	57.6	58.1	1041-1177 bp	1.0	61.2
trnS-GCU-trnS-CGA_p3	AGTCCACTCAGCCATCTCTC	AGGGTTTTCATCAAACGAATCAC	55.0	39.1	58.5	57.9	1037-1173 bp	1.0	60.5
trnS-GCU-trnS-CGA_p4	AGTCCACTCAGCCATCTCTC	GGGTTTTCATCAAACGAATCACA	55.0	39.1	58.5	58.1	1036-1172 bp	1.0	60.4
trnS-GCU-trnS-CGA_p5	GCTTTAGTCCACTCAGCCAT	AGGGTTTTCATCAAACGAATCA	50.0	36.4	57.6	56.5	1042-1178 bp	1.0	58.5