primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	TACTCTGGGACGGAAGGATT	CGAAAACTGACAGCAGCTTG	20	20	50.0	50.0	57.5	58.0	0.0	1.2	0.573131	4878	5879	1106-1274	1.000000	83.8
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	TACTCTGGGACGGAAGGATT	GCACAGGAATGACTGGCATA	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.587530	4878	5879	1060-1228	1.000000	83.8
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	TACTCTGGGACGGAAGGATT	TCGAAAACTGACAGCAGCTT	20	20	50.0	45.0	57.5	58.0	0.0	1.2	0.591285	4878	5879	1107-1275	1.000000	83.7
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	GACCAAAACCCGTTGCCTTA	CGAAAACTGACAGCAGCTTG	20	20	50.0	50.0	58.7	58.0	0.0	1.2	0.707780	4878	5879	1068-1236	1.000000	82.9
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	TACTCTGGGACGGAAGGATT	ATCGAAAACTGACAGCAGCT	20	20	50.0	45.0	57.5	57.8	0.0	1.2	0.720721	4878	5879	1108-1276	1.000000	82.6
trnS-GCU-trnS-CGA_p1	trnS-GCU-trnS-CGA	1	GCTTTAGTCCACTCAGCCAT	AGGGTTTTCATCAAACGAATCAC	20	23	50.0	39.1	57.6	57.9	0.9	0.0	3.531169	6857	7824	1042-1178	1.000000	61.3
trnS-GCU-trnS-CGA_p2	trnS-GCU-trnS-CGA	2	GCTTTAGTCCACTCAGCCAT	GGGTTTTCATCAAACGAATCACA	20	23	50.0	39.1	57.6	58.1	0.9	0.0	3.544172	6857	7824	1041-1177	1.000000	61.2
trnS-GCU-trnS-CGA_p3	trnS-GCU-trnS-CGA	3	AGTCCACTCAGCCATCTCTC	AGGGTTTTCATCAAACGAATCAC	20	23	55.0	39.1	58.5	57.9	0.0	0.0	3.637183	6857	7824	1037-1173	1.000000	60.5
trnS-GCU-trnS-CGA_p4	trnS-GCU-trnS-CGA	4	AGTCCACTCAGCCATCTCTC	GGGTTTTCATCAAACGAATCACA	20	23	55.0	39.1	58.5	58.1	0.0	0.0	3.650185	6857	7824	1036-1172	1.000000	60.4
trnS-GCU-trnS-CGA_p5	trnS-GCU-trnS-CGA	5	GCTTTAGTCCACTCAGCCAT	AGGGTTTTCATCAAACGAATCA	20	22	50.0	36.4	57.6	56.5	0.9	0.0	3.877005	6857	7824	1042-1178	1.000000	58.5
atpH-atpI_p1	atpH-atpI	1	AACAGAAGCGGCAGAAATCA	CCTGTCATGTTCCTTGGGTT	20	20	45.0	50.0	58.1	57.7	1.4	0.0	0.396609	12818	13334	610-657	1.000000	82.7
atpH-atpI_p2	atpH-atpI	2	AACAGAAGCGGCAGAAATCA	CTTTGCAACTTTAGCTGCGG	20	20	45.0	50.0	58.1	58.3	1.4	0.0	0.413821	12818	13334	563-610	1.000000	82.7
atpH-atpI_p3	atpH-atpI	3	AACAGAAGCGGCAGAAATCA	CAACTTTAGCTGCGGCCTAT	20	20	45.0	50.0	58.1	58.3	1.4	15.5	0.435361	12818	13334	558-605	1.000000	82.5
atpH-atpI_p4	atpH-atpI	4	CAGCAGCAATAACAGAAGCG	CCTGTCATGTTCCTTGGGTT	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.462491	12818	13334	620-667	1.000000	82.1
atpH-atpI_p5	atpH-atpI	5	CAGCAGCAATAACAGAAGCG	CTTTGCAACTTTAGCTGCGG	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.479704	12818	13334	573-620	1.000000	82.1
atpI-rps2_p1	atpI-rps2	1	GGAACCCACGGACCATATTC	ATTTCGATTCCGGCGAATGA	20	20	55.0	45.0	58.0	58.1	0.0	9.3	0.088724	14079	14254	571-577	1.000000	82.5
atpI-rps2_p2	atpI-rps2	2	GGAACCCACGGACCATATTC	TTTCGATTCCGGCGAATGAT	20	20	55.0	45.0	58.0	58.1	0.0	9.3	0.088724	14079	14254	570-576	1.000000	82.5
atpI-rps2_p3	atpI-rps2	3	GGAACCCACGGACCATATTC	AATTGTGATCCCGATCTCGC	20	20	55.0	50.0	58.0	58.1	0.0	6.3	0.165748	14079	14254	595-601	1.000000	81.9
atpI-rps2_p4	atpI-rps2	4	CCCCTGACCAGTTAGAAACG	ATTTCGATTCCGGCGAATGA	20	20	55.0	45.0	57.9	58.1	0.0	9.3	0.146658	14079	14254	621-627	1.000000	81.8
atpI-rps2_p5	atpI-rps2	5	CCCCTGACCAGTTAGAAACG	TTTCGATTCCGGCGAATGAT	20	20	55.0	45.0	57.9	58.1	0.0	9.3	0.146658	14079	14254	620-626	1.000000	81.8
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	29455	30114	764-1145	1.000000	85.0
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	29455	30114	801-1182	1.000000	84.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	29455	30114	729-1110	1.000000	84.4
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.262716	29455	30114	766-1147	1.000000	84.3
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	29455	30114	798-1179	1.000000	83.7
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.8	58.0	0.0	4.2	0.208039	35434	35593	225-244	1.000000	86.0
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.195421	35434	35593	261-280	1.000000	85.7
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.348744	35434	35593	223-242	1.000000	85.0
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.394901	35434	35593	246-265	1.000000	84.3
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.394901	35434	35593	245-264	1.000000	84.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	45592	46476	923-953	1.000000	84.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	58.2	58.2	0.0	10.2	0.406999	45592	46476	954-984	1.000000	84.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	45592	46476	917-947	1.000000	83.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAAAGCCAGCTATCGGAGTC	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.7	58.2	0.0	10.2	0.565978	45592	46476	969-999	1.000000	82.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GAAAAGCCAGCTATCGGAGT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.7	58.2	0.0	10.2	0.565978	45592	46476	970-1000	1.000000	82.7
psaJ-rpl33_p1	psaJ-rpl33	1	TCTCTGTGGCACCGGTATTA	TACCCGGACATCTTTCCCTT	20	20	50.0	50.0	58.1	58.0	16.3	0.7	0.183572	62777	63266	617-640	1.000000	84.9
psaJ-rpl33_p2	psaJ-rpl33	2	TCTCTGTGGCACCGGTATTA	TTACCCGGACATCTTTCCCT	20	20	50.0	50.0	58.1	58.0	16.3	0.7	0.183572	62777	63266	618-641	1.000000	84.9
psaJ-rpl33_p3	psaJ-rpl33	3	TGGTTCGGTTCTTTAGCAGG	TACCCGGACATCTTTCCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.7	0.217779	62777	63266	588-611	1.000000	84.9
psaJ-rpl33_p4	psaJ-rpl33	4	CATATCTCTCTGTGGCACCG	TACCCGGACATCTTTCCCTT	20	20	55.0	50.0	57.9	58.0	16.3	0.7	0.184825	62777	63266	623-646	1.000000	84.8
psaJ-rpl33_p5	psaJ-rpl33	5	CATATCTCTCTGTGGCACCG	TTACCCGGACATCTTTCCCT	20	20	55.0	50.0	57.9	58.0	16.3	0.7	0.184825	62777	63266	624-647	1.000000	84.8
rpl33-rps18_p1	rpl33-rps18	1	AAATCGACACAATACGCCGA	TAATCAATTCGATCCCCCGG	20	20	45.0	50.0	58.0	57.1	0.0	0.0	0.852214	63468	63877	582-628	1.000000	80.9
rpl33-rps18_p2	rpl33-rps18	2	AATCGACACAATACGCCGAA	TAATCAATTCGATCCCCCGG	20	20	45.0	50.0	58.0	57.1	0.0	0.0	0.852214	63468	63877	581-627	1.000000	80.9
rpl33-rps18_p3	rpl33-rps18	3	CGCCGAATCGATTGGAATTG	TAATCAATTCGATCCCCCGG	20	20	50.0	50.0	58.0	57.1	20.3	0.0	0.874071	63468	63877	568-614	1.000000	80.8
rpl33-rps18_p4	rpl33-rps18	4	AAATCGACACAATACGCCGA	GCAACCGCCTACGAAAAGAT	20	20	45.0	50.0	58.0	58.9	0.0	0.0	0.916662	63468	63877	551-597	1.000000	80.6
rpl33-rps18_p5	rpl33-rps18	5	AATCGACACAATACGCCGAA	GCAACCGCCTACGAAAAGAT	20	20	45.0	50.0	58.0	58.9	0.0	0.0	0.916662	63468	63877	550-596	1.000000	80.6
trnL-CAA-rpl32_p1	trnL-CAA-rpl32	1	GTCTCGCGTGTCTACCATTT	TACGCTTCTTTGGAACTGCC	20	20	50.0	50.0	58.0	58.5	5.8	0.0	0.488422	82993	83646	564-818	1.000000	87.0
trnL-CAA-rpl32_p2	trnL-CAA-rpl32	2	CTCGCGTGTCTACCATTTCA	TACGCTTCTTTGGAACTGCC	20	20	50.0	50.0	58.0	58.5	5.8	0.0	0.489792	82993	83646	562-816	1.000000	87.0
trnL-CAA-rpl32_p3	trnL-CAA-rpl32	3	TACCATTTCACCACCAAGGC	TACGCTTCTTTGGAACTGCC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.562719	82993	83646	552-806	1.000000	86.5
trnL-CAA-rpl32_p4	trnL-CAA-rpl32	4	GTCTCGCGTGTCTACCATTT	GTACGCTTCTTTGGAACTGC	20	20	50.0	50.0	58.0	57.4	5.8	0.0	0.604094	82993	83646	565-819	1.000000	86.0
trnL-CAA-rpl32_p5	trnL-CAA-rpl32	5	CTCGCGTGTCTACCATTTCA	GTACGCTTCTTTGGAACTGC	20	20	50.0	50.0	58.0	57.4	5.8	0.0	0.605464	82993	83646	563-817	1.000000	86.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.8	57.9	14.0	0.0	0.298641	83803	84302	592-647	1.000000	85.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCATTGAAAGCTTTT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.8	57.8	14.0	0.0	0.359368	83803	84302	585-640	1.000000	84.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGCAGCATTGAAAGCTTTT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.8	57.8	14.0	0.0	0.370658	83803	84302	591-646	1.000000	84.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGCAGCATTGAAAGCTTTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.8	57.8	14.0	0.0	0.370658	83803	84302	590-645	1.000000	84.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCATTGAAAGCTTTT	GAGCAGCGTGTCTACCAATT	20	20	45.0	50.0	57.8	58.3	14.0	0.0	0.447510	83803	84302	586-641	1.000000	84.2
psaC-rps15_p1	psaC-rps15	1	ACATCCGTCCCAAGGTATCA	AATTTTGGGAAAACGGCAAC	20	20	50.0	40.0	58.1	55.9	0.0	0.0	2.209323	86220	87181	1173-1323	1.000000	66.3
psaC-rps15_p2	psaC-rps15	2	CAAGCTCGGACACATTGAGT	AATTTTGGGAAAACGGCAAC	20	20	50.0	40.0	58.2	55.9	0.0	0.0	2.282647	86220	87181	1133-1283	1.000000	65.9
psaC-rps15_p3	psaC-rps15	3	AATACATCTGTGGGGCAAGC	AATTTTGGGAAAACGGCAAC	20	20	50.0	40.0	58.2	55.9	0.0	0.0	2.315694	86220	87181	1148-1298	1.000000	65.6
psaC-rps15_p4	psaC-rps15	4	CGGACACATTGAGTACACCC	AATTTTGGGAAAACGGCAAC	20	20	55.0	40.0	58.3	55.9	0.0	0.0	2.361441	86220	87181	1127-1277	1.000000	65.3
psaC-rps15_p5	psaC-rps15	5	ACATCCGTCCCAAGGTATCA	AAATTTTGGGAAAACGGCAAC	20	21	50.0	38.1	58.1	56.7	0.0	0.0	2.445863	86220	87181	1174-1324	1.000000	64.5
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	88864	89392	521-634	1.000000	86.6
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	TTCGAATCCTACCTGGGGAG	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.2	57.8	0.0	18.9	0.426384	88864	89392	511-624	1.000000	86.3
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.483095	88864	89392	525-638	1.000000	85.7
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CTCAGTAGCTCAGCGGTAGA	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.3	57.8	0.0	18.9	0.546303	88864	89392	561-674	1.000000	84.9
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TCAGTAGCTCAGCGGTAGAG	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.3	57.8	0.0	18.9	0.546303	88864	89392	560-673	1.000000	84.9
trnI-GAU-rrn16S_p1	trnI-GAU-rrn16S	1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.2	57.7	0.0	7.8	0.485056	95022	95366	435-458	1.000000	82.8
trnI-GAU-rrn16S_p2	trnI-GAU-rrn16S	2	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	20	20	55.0	55.0	57.8	57.7	0.0	7.8	0.462074	95022	95366	467-490	1.000000	82.5
trnI-GAU-rrn16S_p3	trnI-GAU-rrn16S	3	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	57.8	57.7	4.7	7.8	0.464916	95022	95366	486-509	1.000000	82.2
trnI-GAU-rrn16S_p4	trnI-GAU-rrn16S	4	CTATCCATGTGGCTGAGAGC	AGTGACTGGAGTGAAGTCGT	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.473195	95022	95366	489-512	1.000000	82.1
trnI-GAU-rrn16S_p5	trnI-GAU-rrn16S	5	CGCTGATGAGCACATTGAAC	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	57.8	58.3	4.7	0.0	0.476037	95022	95366	508-531	1.000000	81.9
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	AAGTGACACTCTACCGCTGA	TCGTACTCTGGCAACTTTACG	20	21	50.0	47.6	58.4	58.1	8.9	9.9	1.445086	97119	98693	1607-1660	1.000000	76.7
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	TGACACTCTACCGCTGAGTT	TCGTACTCTGGCAACTTTACG	20	21	50.0	47.6	58.4	58.1	0.0	9.9	1.445086	97119	98693	1604-1657	1.000000	76.7
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	TCAAAGTGACACTCTACCGC	TCGTACTCTGGCAACTTTACG	20	21	50.0	47.6	57.6	58.1	8.9	9.9	1.496277	97119	98693	1610-1663	1.000000	76.3
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	AAAGTGACACTCTACCGCTG	TCGTACTCTGGCAACTTTACG	20	21	50.0	47.6	57.6	58.1	8.9	9.9	1.502948	97119	98693	1608-1661	1.000000	76.2
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CAAAGTGACACTCTACCGCT	TCGTACTCTGGCAACTTTACG	20	21	50.0	47.6	57.6	58.1	8.9	9.9	1.502948	97119	98693	1609-1662	1.000000	76.2
rps7-trnL-CAA_p1	rps7-trnL-CAA	1	ATGGCAGAGGCAAATAGAGC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.029376	100009	100768	578-1015	1.000000	91.8
rps7-trnL-CAA_p2	rps7-trnL-CAA	2	ATGGCAGAGGCAAATAGAGC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.030746	100009	100768	576-1013	1.000000	91.8
rps7-trnL-CAA_p3	rps7-trnL-CAA	3	GGCAGAGGCAAATAGAGCTT	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.057719	100009	100768	576-1013	1.000000	91.5
rps7-trnL-CAA_p4	rps7-trnL-CAA	4	GGCAGAGGCAAATAGAGCTT	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.059089	100009	100768	574-1011	1.000000	91.5
rps7-trnL-CAA_p5	rps7-trnL-CAA	5	ATGGCAGAGGCAAATAGAGC	TACCATTTCACCACCAAGGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.103674	100009	100768	566-1003	1.000000	91.2