Hotspot

clpP

LSC · 77140-79220 · 2081 bp

Pi 0.0639

MarkerSeek score 73.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2081
variable_sites: 132
pi: 0.0639
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGACATTTCGGATTGGCTGT	TGGACAGAAAGTAAACGGCC	45.0	50.0	57.8	58.1	2986 bp	0.5	57.5
clpP_p2	CTAGTACATGTTCCTCGGCG	ACCAAGGCAAACCCATGAAA	55.0	45.0	57.9	58.2	2986 bp	0.5	57.4
clpP_p3	TGACATTTCGGATTGGCTGT	AAACGGCCGGGATCATTTAA	45.0	45.0	57.8	57.9	2974 bp	0.5	57.4
clpP_p4	CTAGTACATGTTCCTCGGCG	AATACGCAATGGTGAAGGGG	55.0	50.0	57.9	58.2	2772 bp	0.5	57.1
clpP_p5	CTAGTACATGTTCCTCGGCG	CGATACCAAGGCAAACCCAT	55.0	50.0	57.9	58.2	2990 bp	0.5	57.1