Hotspot

rpl23-rpl2

IRa · 162624-162641 · 18 bp

Back to Zygia

Pi 0.0556
MarkerSeek score 72.50
Primer available yes
Label rpl23-rpl2

Feature Metadata

label_name
rpl23-rpl2
feature_type
igs
region
IRa
length_bp
18
variable_sites
1
pi
0.0556
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-rpl2_p1 CGCTTCAACCGGGTTATTCT CACTTGACTGTCTACGGCTC 50.0 55.0 58.3 58.0 134 bp 1.0 78.3
rpl23-rpl2_p2 CGCTTCAACCGGGTTATTCT GACTGTCTACGGCTCCATTG 50.0 55.0 58.3 58.1 129 bp 1.0 77.9
rpl23-rpl2_p3 ATCATTACGCTTCAACCGGG CACTTGACTGTCTACGGCTC 50.0 55.0 58.3 58.0 141 bp 1.0 77.7
rpl23-rpl2_p4 ATCATTACGCTTCAACCGGG GACTGTCTACGGCTCCATTG 50.0 55.0 58.3 58.1 136 bp 1.0 77.3
rpl23-rpl2_p5 CGCTTCAACCGGGTTATTCT TGCTCGGGGTAGAAGTTTTG 50.0 50.0 58.3 57.8 105 bp 1.0 77.2