Hotspot

rpl22-rps19

LSC · 91698-91867 · 170 bp

Back to Zygia

Pi 0.0701
MarkerSeek score 73.20
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
170
variable_sites
11
pi
0.0701
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TTGTCCCATAATCCCCCACT ATAAACTTCCGGGGACATGC 50.0 50.0 58.0 57.9 792-810 bp 1.0 85.8
rpl22-rps19_p2 TTGTCCCATAATCCCCCACT GGGCCCCAATTGGTAGAAAA 50.0 50.0 58.0 58.1 1215-1227 bp 1.0 85.3
rpl22-rps19_p3 TTGTCCCATAATCCCCCACT TTGATTCTTCGTCGCCGTAG 50.0 50.0 58.0 58.1 1123-1135 bp 1.0 85.1
rpl22-rps19_p4 TTGTCCCATAATCCCCCACT AAAAGTTTGGGTAGAGCCGG 50.0 50.0 58.0 58.1 1321-1333 bp 1.0 84.8
rpl22-rps19_p5 TTGTCCCATAATCCCCCACT TCGTCGCCGTAGTAAGTAGT 50.0 50.0 58.0 58.0 1115 bp 0.5 59.9