Hotspot

rps8-rpl14

LSC · 87967-88263 · 297 bp

Back to Zygia

Pi 0.0505
MarkerSeek score 81.30
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
297
variable_sites
15
pi
0.0505
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 GCAATGGTGTCCTTACCCAT GCTCGGGAATTGAGACAGTT 50.0 50.0 57.9 57.9 380-405 bp 1.0 81.1
rps8-rpl14_p2 GATGGTATTCGAACCGTCCC GCTCGGGAATTGAGACAGTT 55.0 50.0 58.1 57.9 443-468 bp 1.0 80.7
rps8-rpl14_p3 GCAATGGTGTCCTTACCCAT AATTTTTGGTGCGATCGCTC 50.0 45.0 57.9 57.7 396-421 bp 1.0 79.7
rps8-rpl14_p4 GATGGTATTCGAACCGTCCC AATTTTTGGTGCGATCGCTC 55.0 45.0 58.1 57.7 459-484 bp 1.0 79.3
rps8-rpl14_p5 GATGGTATTCGAACCGTCCC GAATTTTTGGTGCGATCGCT 55.0 45.0 58.1 57.7 460-485 bp 1.0 79.3