Hotspot

atpF-atpH

LSC · 14389-15096 · 708 bp

Back to Zygia

Pi 0.0284
MarkerSeek score 75.00
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
708
variable_sites
20
pi
0.0284
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG TGGGCTGGTTGTAGCATTAG 50.0 50.0 57.9 57.6 826-842 bp 1.0 78.0
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG AGGCAGAGGGAAAAATACGG 50.0 50.0 57.9 57.6 893-909 bp 1.0 77.8
atpF-atpH_p3 ATTAAACCCGAAACTCCCGG GCAGAGGGAAAAATACGGGG 50.0 55.0 57.9 58.6 891-907 bp 1.0 76.3
atpF-atpH_p4 ATTAAACCCGAAACTCCCGG GGCAGAGGGAAAAATACGGG 50.0 55.0 57.9 58.6 892-908 bp 1.0 76.3
atpF-atpH_p5 ATTAAACCCGAAACTCCCGG CCGAGGCAGAGGGAAAAATA 50.0 50.0 57.9 57.3 896-912 bp 1.0 75.5