Hotspot

trnQ-UUG-psbK

LSC · 7458-8049 · 592 bp

Back to Zygia

Pi 0.055
MarkerSeek score 76.10
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
592
variable_sites
32
pi
0.055
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 ATATGCTCTCTCTGGGACGG AGCAGCTTGCCAAACAAAAG 55.0 45.0 58.4 58.1 846 bp 0.5 56.6
trnQ-UUG-psbK_p2 ATATGCTCTCTCTGGGACGG CTTACAGCAGCTTGCCAAAC 55.0 50.0 58.4 57.9 851 bp 0.5 56.6
trnQ-UUG-psbK_p3 CTCTCTGGGACGGAAGGATT AGCAGCTTGCCAAACAAAAG 55.0 45.0 58.5 58.1 839 bp 0.5 55.5
trnQ-UUG-psbK_p4 CTCTCTGGGACGGAAGGATT CTTACAGCAGCTTGCCAAAC 55.0 50.0 58.5 57.9 844 bp 0.5 55.5
trnQ-UUG-psbK_p5 TCTCTGGGACGGAAGGATTC AGCAGCTTGCCAAACAAAAG 55.0 45.0 58.5 58.1 838 bp 0.5 55.4