Hotspot

rps16-trnQ-UUG

LSC · 6684-7385 · 702 bp

Back to Zygia

Pi 0.0608
MarkerSeek score 82.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
702
variable_sites
40
pi
0.0608
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 782-784 bp 1.0 87.4
rps16-trnQ-UUG_p2 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 770-772 bp 1.0 86.0
rps16-trnQ-UUG_p3 TGTCTTTCAAGTCGCACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 783-785 bp 1.0 84.7
rps16-trnQ-UUG_p4 GTCTTTCAAGTCGCACGTTG AAATAAAAATGGGGCGTGGC 50.0 45.0 58.0 57.6 839-841 bp 1.0 84.6
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTTTAC GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 774-776 bp 1.0 84.5