Hotspot

petB

LSC · 72099-73532 · 1434 bp

Back to Zoysia

Pi 0.00107
MarkerSeek score 58.20
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1434
variable_sites
4
pi
0.00107
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 GGTAGTTCGACCGTGGATTT ACCACAACCTACCCGATATGA 50.0 47.6 57.6 58.2 1531-1537 bp 1.0 67.5
petB_p2 GGTAGTTCGACCGTGGATTT ACCACAACCTACCCGATATG 50.0 50.0 57.6 56.7 1531-1537 bp 1.0 67.1
petB_p3 AGGTAGTTCGACCGTGGATT ACCACAACCTACCCGATATGA 50.0 47.6 58.4 58.2 1532-1538 bp 1.0 66.9
petB_p4 GGTAGTTCGACCGTGGATTT TGAAATACCACAACCTACCCG 50.0 47.6 57.6 57.7 1537-1543 bp 1.0 66.8
petB_p5 AGGTAGTTCGACCGTGGATT ACCACAACCTACCCGATATG 50.0 50.0 58.4 56.7 1532-1538 bp 1.0 66.6