Hotspot

rpl32-trnL-UAG

SSC · 105491-106015 · 525 bp

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Pi 0.0136
MarkerSeek score 50.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
525
variable_sites
12
pi
0.0136
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAGCCAAAACCAAAAGGTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 610-620 bp 1.0 80.3
rpl32-trnL-UAG_p2 GCAGCCAAAACCAAAAGGTT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.0 57.8 603-613 bp 1.0 79.8
rpl32-trnL-UAG_p3 GCAGCCAAAACCAAAAGGTT GCTTGAGCACTGCTTCCTAA 45.0 50.0 58.0 58.2 624-634 bp 1.0 79.7
rpl32-trnL-UAG_p4 GCAGCCAAAACCAAAAGGTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.0 57.8 609-619 bp 1.0 79.7
rpl32-trnL-UAG_p5 GCAGCCAAAACCAAAAGGTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.0 57.8 608-618 bp 1.0 79.7