Hotspot

rbcL-psaI

LSC · 57165-58039 · 875 bp

Back to Zoysia

Pi 0.0032
MarkerSeek score 48.50
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
875
variable_sites
6
pi
0.0032
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 CACATTCGATTTCAAGCCGG CCATTGCAATTGCCGGAAAT 50.0 45.0 57.8 58.0 979 bp 1.0 78.8
rbcL-psaI_p2 ACATTCGATTTCAAGCCGGT CCATTGCAATTGCCGGAAAT 45.0 45.0 58.2 58.0 978 bp 1.0 78.8
rbcL-psaI_p3 CACATTCGATTTCAAGCCGG AAGAAGCCATTGCAATTGCC 50.0 45.0 57.8 57.9 985 bp 1.0 78.2
rbcL-psaI_p4 ACATTCGATTTCAAGCCGGT AAGAAGCCATTGCAATTGCC 45.0 45.0 58.2 57.9 984 bp 1.0 78.2
rbcL-psaI_p5 CGCAGCTTGTGAAGTATGGA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.3 58.0 1006 bp 1.0 78.1