Hotspot

atpB-rbcL

LSC · 54928-55736 · 809 bp

Back to Zoysia

Pi 0.00874
MarkerSeek score 67.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
809
variable_sites
14
pi
0.00874
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TTTGATCAATACGCCCTGCA GCTTGAAATCCAACACCTGC 45.0 50.0 57.9 57.9 919-929 bp 1.0 81.2
atpB-rbcL_p2 TTTGATCAATACGCCCTGCA TTCGTATTCCGGGGTGTAGT 45.0 50.0 57.9 58.1 965-975 bp 1.0 80.4
atpB-rbcL_p3 TATCCAGCACGGGTCCAATA GCTTGAAATCCAACACCTGC 50.0 50.0 58.2 57.9 940-950 bp 1.0 80.4
atpB-rbcL_p4 CCAGCACGGGTCCAATAATT GCTTGAAATCCAACACCTGC 50.0 50.0 58.2 57.9 937-947 bp 1.0 80.2
atpB-rbcL_p5 CCGGGGGAAAAGTGATATCC GCTTGAAATCCAACACCTGC 55.0 50.0 57.7 57.9 955-965 bp 1.0 79.8