Hotspot

ndhC-trnV-UAC

LSC · 51044-51984 · 941 bp

Back to Zoysia

Pi 0.0012
MarkerSeek score 53.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
941
variable_sites
2
pi
0.0012
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGTCCTTCATTAATCGGGGC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.4 57.9 1073-1079 bp 1.0 76.7
ndhC-trnV-UAC_p2 GGTCCTTCATTAATCGGGGC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.4 57.9 1166-1172 bp 1.0 76.4
ndhC-trnV-UAC_p3 GGTCCTTCATTAATCGGGGC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.4 58.3 1074-1080 bp 1.0 74.8
ndhC-trnV-UAC_p4 GTCCTTCATTAATCGGGGCT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.4 57.9 1072-1078 bp 1.0 74.7
ndhC-trnV-UAC_p5 GTCCTTCATTAATCGGGGCT AGCCCAATGCAGAAAAATGC 50.0 45.0 57.4 57.9 1165-1171 bp 1.0 74.5