Hotspot

trnT-UGU-trnL-UAA

LSC · 46959-47764 · 806 bp

Back to Zoysia

Pi 0.000827
MarkerSeek score 53.80
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
806
variable_sites
2
pi
0.000827
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 904-907 bp 1.0 80.4
trnT-UGU-trnL-UAA_p2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.1 58.0 896-899 bp 1.0 80.0
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 55.0 50.0 57.9 58.0 874-877 bp 1.0 79.5
trnT-UGU-trnL-UAA_p4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.0 58.2 894-897 bp 1.0 78.4
trnT-UGU-trnL-UAA_p5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 50.0 50.0 58.0 57.7 903-906 bp 1.0 78.2