Hotspot

trnR-UCU-trnfM-CAU

LSC · 37140-37270 · 131 bp

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Pi 0.0107
MarkerSeek score 54.50
Primer available yes
Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name
trnR-UCU-trnfM-CAU
feature_type
igs
region
LSC
length_bp
131
variable_sites
4
pi
0.0107
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnfM-CAU_p1 AGAAGACCTCTGTCCTATCCA ATGGTAGAGGGCGTATCCAA 47.6 50.0 56.9 57.9 284-287 bp 1.0 64.8
trnR-UCU-trnfM-CAU_p2 AGAAGACCTCTGTCCTATCCA AGGGCGTATCCAACTCTCTT 47.6 50.0 56.9 58.1 277-280 bp 1.0 64.5
trnR-UCU-trnfM-CAU_p3 AGGTTTAGAAGACCTCTGTCCT ATGGTAGAGGGCGTATCCAA 45.5 50.0 57.9 57.9 290-293 bp 1.0 64.1
trnR-UCU-trnfM-CAU_p4 AGAAGACCTCTGTCCTATCCA GCTAGTTCCCCGCTCTTTAG 47.6 55.0 56.9 57.8 251-254 bp 1.0 64.0
trnR-UCU-trnfM-CAU_p5 AGGTTTAGAAGACCTCTGTCCT AGGGCGTATCCAACTCTCTT 45.5 50.0 57.9 58.1 283-286 bp 1.0 63.8