Hotspot

atpI-atpH

LSC · 32415-33226 · 812 bp

Back to Zoysia

Pi 0.00197
MarkerSeek score 50.20
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
812
variable_sites
4
pi
0.00197
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TAGCCGCAGCCTATATAGGT CTCTACAGCTTGTCCTGCAG 50.0 55.0 57.5 58.0 967 bp 1.0 76.1
atpI-atpH_p2 ATTTTTGCAACGTTAGCCGC CTCTACAGCTTGTCCTGCAG 45.0 55.0 58.6 58.0 980 bp 1.0 75.6
atpI-atpH_p3 TAGCCGCAGCCTATATAGGT GCAGTACCTTGACCAACTCC 50.0 55.0 57.5 58.2 948 bp 1.0 74.6
atpI-atpH_p4 TAGCCGCAGCCTATATAGGT AAGCAGCAGCAATTAGTGGA 50.0 45.0 57.5 57.8 881 bp 1.0 74.5
atpI-atpH_p5 TAGCCGCAGCCTATATAGGT GCCAATCCAGCAGCAATAAC 50.0 50.0 57.5 57.8 903 bp 1.0 74.4