Hotspot

trnG-UCC-trnT-GGU

LSC · 13876-14881 · 1006 bp

Back to Zoysia

Pi 0.00119
MarkerSeek score 47.20
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1006
variable_sites
3
pi
0.00119
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TACCGCTGAGTTAAAAGGGC 38.1 50.0 55.5 57.9 1060-1061 bp 1.0 51.1
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT ACTCTACCACTGGGGGAAAA 38.1 50.0 55.5 57.9 1131-1132 bp 1.0 50.9
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT GGCATTACTCTACCGCTGAG 38.1 55.0 55.5 57.9 1070-1071 bp 1.0 50.8
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC TACCGCTGAGTTAAAAGGGC 39.1 50.0 57.3 57.9 1063-1064 bp 1.0 50.0
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT TACCGCTGAGTTAAAAGGGC 39.1 50.0 57.3 57.9 1062-1063 bp 1.0 50.0