Hotspot

matK-trnK-UUU

LSC · 3218-3889 · 672 bp

Back to Zoysia

Pi 0.000794
MarkerSeek score 47.10
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
672
variable_sites
1
pi
0.000794
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TTCCTGAAAGAGAAGTGGGT TGGGTTGCTAACTCAATGGT 45.0 45.0 56.0 57.0 786-788 bp 1.0 56.4
matK-trnK-UUU_p2 TTCCTGAAAGAGAAGTGGGT TCAATGGTAGAGTACTCGGCT 45.0 47.6 56.0 58.0 774-776 bp 1.0 56.0
matK-trnK-UUU_p3 TTCCTGAAAGAGAAGTGGGT CGATTTTTGGATTGCGATCGT 45.0 42.9 56.0 57.9 827-829 bp 1.0 55.7
matK-trnK-UUU_p4 TTCCTGAAAGAGAAGTGGGT TCTGGGTTGCTAACTCAATGG 45.0 47.6 56.0 57.9 788-790 bp 1.0 55.5
matK-trnK-UUU_p5 TTCCTGAAAGAGAAGTGGGT CTGGGTTGCTAACTCAATGGT 45.0 47.6 56.0 58.2 787-789 bp 1.0 54.5