Hotspot

rpl22

LSC · 83086-83250 · 165 bp

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Pi 0.0788

MarkerSeek score 65.70

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 165
variable_sites: 31
pi: 0.0788
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GCGTTCGACCCTCCATTAAT	ATCCGATATGGCACGTTCAC	50.0	50.0	58.1	58.1	1107-1128 bp	1.0	89.7
rpl22_p2	GCGTTCGACCCTCCATTAAT	GATCCACTTGGCTACATCCG	50.0	55.0	58.1	58.1	1153-1174 bp	1.0	89.5
rpl22_p3	AGCGCGAATAGTTTGTAGGG	ATCCGATATGGCACGTTCAC	50.0	50.0	58.1	58.1	1412-1433 bp	1.0	88.3
rpl22_p4	AGCGCGAATAGTTTGTAGGG	GATCCACTTGGCTACATCCG	50.0	55.0	58.1	58.1	1458-1479 bp	1.0	88.0
rpl22_p5	GCGTTCGACCCTCCATTAAT	ACAGCAGCTAAGCTAGGTCT	50.0	50.0	58.1	58.1	857 bp	0.4	54.5