Hotspot

rpl32-ndhF

SSC · 118784-119529 · 746 bp

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Pi 0.0335

MarkerSeek score 67.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 746
variable_sites: 56
pi: 0.0335
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AGCTAATAATCGCTGACGCT	TGCGGATTTAGCAATAGCCC	45.0	50.0	57.2	58.4	2025-2206 bp	1.0	79.5
rpl32-ndhF_p2	AGCTAATAATCGCTGACGCT	GTCGAGTGAACCAAAAGCCT	45.0	50.0	57.2	58.4	1804-1985 bp	1.0	79.5
rpl32-ndhF_p3	AGCTAATAATCGCTGACGCT	TGAAGAGGAAATTGTGCGGA	45.0	45.0	57.2	57.4	2039-2220 bp	1.0	78.1
rpl32-ndhF_p4	AGCTAATAATCGCTGACGCT	CCAAAGTCTCCTACGCGATT	45.0	50.0	57.2	57.7	2026-2043 bp	0.8	65.0
rpl32-ndhF_p5	CTGACGCTTGCCCAAAATTA	CCAAAGTCTCCTACGCGATT	45.0	50.0	57.0	57.7	2014-2031 bp	0.8	63.4