Hotspot

rpl16-rps3

LSC · 82137-82295 · 159 bp

Back to Zostera

Pi 0.079

MarkerSeek score 70.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 25
pi: 0.079
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CTGAACTCTGCAATGGAGCT	ATTAATGGAGGGTCGAACGC	50.0	50.0	57.9	58.1	972-1008 bp	1.0	86.9
rpl16-rps3_p2	GGTTCCGTCGTTCCTATAGC	ATTAATGGAGGGTCGAACGC	55.0	50.0	57.9	58.1	1012-1048 bp	1.0	86.5
rpl16-rps3_p3	CTGAACTCTGCAATGGAGCT	CCCTACAAACTATTCGCGCT	50.0	50.0	57.9	58.1	667-703 bp	0.8	76.0
rpl16-rps3_p4	AATGGGTTCCTGGTTCGTTC	CCCTACAAACTATTCGCGCT	50.0	50.0	58.1	58.1	785-821 bp	0.8	75.8
rpl16-rps3_p5	AATGGGTTCCTGGTTCGTTC	ATTAATGGAGGGTCGAACGC	50.0	50.0	58.1	58.1	1090-1126 bp	0.8	74.7