Hotspot

psbH-petB

LSC · 74468-74572 · 105 bp

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Pi 0.0495

MarkerSeek score 70.90

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 105
variable_sites: 12
pi: 0.0495
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	TATGGCTACACGAACCGTTG	GTACGGCTCAAGCAGAAACA	50.0	50.0	58.0	58.5	1005-1036 bp	1.0	80.8
psbH-petB_p2	TATGGCTACACGAACCGTTG	TACGGCTCAAGCAGAAACAC	50.0	50.0	58.0	58.5	1004-1035 bp	1.0	80.8
psbH-petB_p3	AGATATGGAGATGGCGACCA	TACGGCTCAAGCAGAAACAC	50.0	50.0	58.0	58.5	1156-1184 bp	1.0	80.2
psbH-petB_p4	ATATGGAGATGGCGACCAGA	GTACGGCTCAAGCAGAAACA	50.0	50.0	58.0	58.5	1155-1165 bp	0.8	72.0
psbH-petB_p5	ATATGGAGATGGCGACCAGA	TACGGCTCAAGCAGAAACAC	50.0	50.0	58.0	58.5	1154-1164 bp	0.8	72.0