Hotspot

petG-trnW-CCA

LSC · 65955-66077 · 123 bp

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Pi 0.0591

MarkerSeek score 74.70

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 16
pi: 0.0591
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	181-195 bp	1.0	88.8
petG-trnW-CCA_p2	GACGTGGTGATCAGTTGGAT	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.6	57.9	178-192 bp	1.0	87.0
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	CCCGATGTCGTAGGTTCAAA	50.0	50.0	57.8	57.6	176-190 bp	1.0	86.7
petG-trnW-CCA_p4	ACAGACGTGGTGATCAGTTG	CTCCAAAACCCGATGTCGTA	50.0	50.0	57.8	57.6	184-198 bp	1.0	86.4
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	TCCAAAACCCGATGTCGTAG	50.0	50.0	57.8	57.6	183-197 bp	1.0	86.4