Hotspot

trnF-GAA-ndhJ

LSC · 47983-48593 · 611 bp

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Pi 0.0288

MarkerSeek score 66.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 41
pi: 0.0288
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GGCTGGCCTTTACGTAAAGA	50.0	50.0	58.1	57.9	700-708 bp	1.0	85.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGGCTGGCCTTTACGTAAAG	50.0	50.0	58.1	57.9	701-709 bp	1.0	85.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	713-721 bp	1.0	85.0
trnF-GAA-ndhJ_p4	CGTTGGGATAGCTCAGTTGG	GGCTGGCCTTTACGTAAAGA	55.0	50.0	58.3	57.9	740-748 bp	1.0	82.7
trnF-GAA-ndhJ_p5	CGTTGGGATAGCTCAGTTGG	AGGCTGGCCTTTACGTAAAG	55.0	50.0	58.3	57.9	741-749 bp	1.0	82.6