Hotspot

trnS-UCC-rps4

LSC · 44792-45056 · 265 bp

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Pi 0.037

MarkerSeek score 67.70

Primer available yes

Label trnS-UCC-rps4

Feature Metadata

label_name: trnS-UCC-rps4
feature_type: igs
region: LSC
length_bp: 265
variable_sites: 24
pi: 0.037
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UCC-rps4_p1	AGGCTTTTGTTTACCGAGGG	AGTCGTAGAATATTATTCCCGTCA	50.0	37.5	58.1	57.1	330-336 bp	1.0	48.7
trnS-UCC-rps4_p2	AGGCTTTTGTTTACCGAGGG	TCGTAGAATATTATTCCCGTCAGA	50.0	37.5	58.1	56.9	328-334 bp	1.0	48.7
trnS-UCC-rps4_p3	AGGCTTTTGTTTACCGAGGG	AGTCGTAGAATATTATTCCCGTCAG	50.0	40.0	58.1	58.1	330-336 bp	1.0	48.6
trnS-UCC-rps4_p4	AGGCTTTTGTTTACCGAGGG	GTCGTAGAATATTATTCCCGTCAGA	50.0	40.0	58.1	58.1	329-335 bp	1.0	48.6
trnS-UCC-rps4_p5	AGGCTTTTGTTTACCGAGGG	GTCGTAGAATATTATTCCCGTCAG	50.0	41.7	58.1	56.8	329-335 bp	1.0	48.6