Hotspot

trnK-UUU-rps16

LSC · 3744-4355 · 612 bp

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Pi 0.0418
MarkerSeek score 72.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
612
variable_sites
59
pi
0.0418
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG CTTGAGCCGTACGAGGAAAA 55.0 50.0 58.0 57.9 1021-1038 bp 1.0 86.7
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG TGAGCCGTACGAGGAAAAAG 55.0 50.0 58.0 57.9 1019-1036 bp 1.0 86.7
trnK-UUU-rps16_p3 TCAGGATCAGTCGTGGTCTT CTTGAGCCGTACGAGGAAAA 50.0 50.0 58.1 57.9 1017-1034 bp 1.0 86.2
trnK-UUU-rps16_p4 TCAGGATCAGTCGTGGTCTT TGAGCCGTACGAGGAAAAAG 50.0 50.0 58.1 57.9 1015-1032 bp 1.0 86.2
trnK-UUU-rps16_p5 TTCAGGATCAGTCGTGGTCT TGAGCCGTACGAGGAAAAAG 50.0 50.0 58.1 57.9 1016-1033 bp 1.0 86.2