Hotspot

rpl32-trnL-UAG

SSC · 114327-115112 · 786 bp

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Pi 0.0679
MarkerSeek score 75.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
786
variable_sites
45
pi
0.0679
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGCAATCAATTTCGACAGGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.8 57.9 859-869 bp 1.0 85.9
rpl32-trnL-UAG_p2 AGAGGGGATATTGGACAGCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.8 57.9 898-908 bp 1.0 85.6
rpl32-trnL-UAG_p3 CGCAATCAATTTCGACAGGG GAAGTAGCAAAGCCTTGGGT 50.0 50.0 57.8 58.1 1339-1355 bp 1.0 84.8
rpl32-trnL-UAG_p4 AGAGGGGATATTGGACAGCA GAAGTAGCAAAGCCTTGGGT 50.0 50.0 57.8 58.1 1378-1394 bp 1.0 84.6
rpl32-trnL-UAG_p5 TGGCAGTTCCAAAGAAACGT GAAGTAGCAAAGCCTTGGGT 45.0 50.0 58.2 58.1 1457 bp 0.5 57.3