Hotspot

trnP-UGG-psaJ

LSC · 67460-68192 · 733 bp

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Pi 0.0609
MarkerSeek score 83.00
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
733
variable_sites
41
pi
0.0609
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 810-835 bp 1.0 85.6
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 809-834 bp 1.0 85.6
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 821-846 bp 1.0 85.5
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 820-845 bp 1.0 85.5
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GTACTTACCACTGGTGCCAC 50.0 55.0 58.1 58.2 823-848 bp 1.0 85.5