Hotspot

trnQ-UUG-rps16

LSC · 56743-57406 · 664 bp

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Pi 0.0518
MarkerSeek score 81.10
Primer available yes
Label trnQ-UUG-rps16

Feature Metadata

label_name
trnQ-UUG-rps16
feature_type
igs
region
LSC
length_bp
664
variable_sites
29
pi
0.0518
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-rps16_p1 GAATCAAAAATGGGGCGTGG GTCTTTCAAGTCGCACGTTG 50.0 50.0 58.0 58.0 702-802 bp 1.0 87.0
trnQ-UUG-rps16_p2 GAGGTTCGAATCCTTCCGTC GTCTTTCAAGTCGCACGTTG 55.0 50.0 58.1 58.0 644-744 bp 1.0 86.7
trnQ-UUG-rps16_p3 GTCGAATCAAAAATGGGGCG GTCTTTCAAGTCGCACGTTG 50.0 50.0 58.1 58.0 705-805 bp 1.0 86.3
trnQ-UUG-rps16_p4 GAATCAAAAATGGGGCGTGG ATGTCTTTCAAGTCGCACGT 50.0 45.0 58.0 58.1 704-804 bp 1.0 85.9
trnQ-UUG-rps16_p5 GAATCAAAAATGGGGCGTGG GCACGTTGCTTTTTACCACA 50.0 45.0 58.0 57.8 690-790 bp 1.0 85.5