Hotspot

atpA-trnR-UCU

LSC · 53016-53292 · 277 bp

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Pi 0.062
MarkerSeek score 77.80
Primer available yes
Label atpA-trnR-UCU

Feature Metadata

label_name
atpA-trnR-UCU
feature_type
igs
region
LSC
length_bp
277
variable_sites
16
pi
0.062
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-trnR-UCU_p1 ACTAGGGAAGCAGAAGACGT GGTTCAAATCCTATTGGACGC 50.0 47.6 58.1 57.3 380-386 bp 1.0 73.2
atpA-trnR-UCU_p2 AAGGAGGCTATTCAGGAGCA GGTTCAAATCCTATTGGACGC 50.0 47.6 58.1 57.3 356-362 bp 1.0 73.1
atpA-trnR-UCU_p3 GGCTATTCAGGAGCAGATGG GGTTCAAATCCTATTGGACGC 55.0 47.6 57.8 57.3 351-357 bp 1.0 72.6
atpA-trnR-UCU_p4 TCAGGAGCAGATGGAACTCT GGTTCAAATCCTATTGGACGC 50.0 47.6 57.8 57.3 345-351 bp 1.0 72.2
atpA-trnR-UCU_p5 CACTAGGGAAGCAGAAGACG GGTTCAAATCCTATTGGACGC 55.0 47.6 57.7 57.3 381-387 bp 1.0 71.5