Hotspot

atpH-atpF

LSC · 49552-50032 · 481 bp

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Pi 0.0491
MarkerSeek score 87.00
Primer available yes
Label atpH-atpF

Feature Metadata

label_name
atpH-atpF
feature_type
igs
region
LSC
length_bp
481
variable_sites
23
pi
0.0491
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
87.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpF_p1 AGCACTTTTATTTGCGAATCCC GATGGCCAGTAACCCAAACA 40.9 50.0 57.9 58.1 559-562 bp 1.0 67.6
atpH-atpF_p2 GCACTTTTATTTGCGAATCCCT GATGGCCAGTAACCCAAACA 40.9 50.0 57.9 58.1 558-561 bp 1.0 67.6
atpH-atpF_p3 AGCACTTTTATTTGCGAATCCC TGGCCAGTAACCCAAACAAA 40.9 45.0 57.9 57.8 557-560 bp 1.0 67.0
atpH-atpF_p4 GCACTTTTATTTGCGAATCCCT TGGCCAGTAACCCAAACAAA 40.9 45.0 57.9 57.8 556-559 bp 1.0 67.0
atpH-atpF_p5 CCAGAGGCAGAAGGAAAAAT GATGGCCAGTAACCCAAACA 45.0 50.0 55.6 58.1 648-651 bp 1.0 64.6