Hotspot

atpI-atpH

LSC · 47937-49305 · 1369 bp

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Pi 0.0427
MarkerSeek score 73.30
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1369
variable_sites
56
pi
0.0427
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TACAAGCGGTATTCAAGCCC GGGAAGGAATAAGGCAAGCA 50.0 50.0 58.0 57.9 1900-1901 bp 1.0 83.8
atpI-atpH_p2 TACAAGCGGTATTCAAGCCC AAATCAGTCCTTCCCTTGCG 50.0 50.0 58.0 58.2 1854-1855 bp 1.0 83.7
atpI-atpH_p3 ATTTTTGCTACTTTGGCGGC GGGAAGGAATAAGGCAAGCA 45.0 50.0 57.9 57.9 1878-1879 bp 1.0 83.5
atpI-atpH_p4 ATTTTTGCTACTTTGGCGGC AAATCAGTCCTTCCCTTGCG 45.0 50.0 57.9 58.2 1832-1833 bp 1.0 83.4
atpI-atpH_p5 AACCATTCCCACTTTCGGTC GGGAAGGAATAAGGCAAGCA 50.0 50.0 58.1 57.9 2380-2381 bp 1.0 82.4