Hotspot

trnT-GGU-trnE-UUC

LSC · 30705-31375 · 671 bp

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Pi 0.0408
MarkerSeek score 82.70
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
671
variable_sites
26
pi
0.0408
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GAACCGATGACTTACGCCTT AGAGAGATGTCCTGGACCAC 50.0 55.0 58.0 58.1 734-760 bp 1.0 85.3
trnT-GGU-trnE-UUC_p2 GAACCGATGACTTACGCCTT GAGAGATGTCCTGGACCACT 50.0 55.0 58.0 58.1 733-759 bp 1.0 85.3
trnT-GGU-trnE-UUC_p3 CCATGGCGTTACTCTACCAC GAGAGATGTCCTGGACCACT 55.0 55.0 58.1 58.1 712-738 bp 1.0 85.0
trnT-GGU-trnE-UUC_p4 GAACCGATGACTTACGCCTT CGTTGCCTCCTTGAAAGAGA 50.0 50.0 58.0 57.8 749-775 bp 1.0 84.9
trnT-GGU-trnE-UUC_p5 GAACCGATGACTTACGCCTT TTTCGTAGTACCCTACCCCC 50.0 55.0 58.0 57.8 786-812 bp 1.0 84.7