Hotspot

ycf3-psaA

LSC · 19188-20141 · 954 bp

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Pi 0.0479
MarkerSeek score 73.20
Primer available yes
Label ycf3-psaA

Feature Metadata

label_name
ycf3-psaA
feature_type
igs
region
LSC
length_bp
954
variable_sites
37
pi
0.0479
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-psaA_p1 TTGGTTGAAGATCACAGGGC CTTCTGGTTCCGGCGAAC 50.0 61.1 58.1 58.4 854-1013 bp 1.0 66.9
ycf3-psaA_p2 AAGCTATAGCCCTTACCCCC CTTCTGGTTCCGGCGAAC 55.0 61.1 58.3 58.4 897-1056 bp 1.0 65.5
ycf3-psaA_p3 AAGATCACAGGGCGTTTTGA CTTCTGGTTCCGGCGAAC 45.0 61.1 57.7 58.4 847-1006 bp 1.0 65.5
ycf3-psaA_p4 AGATCACAGGGCGTTTTGAA CTTCTGGTTCCGGCGAAC 45.0 61.1 57.7 58.4 846-1005 bp 1.0 65.5
ycf3-psaA_p5 TGAAGATCACAGGGCGTTTT CTTCTGGTTCCGGCGAAC 45.0 61.1 57.7 58.4 849-1008 bp 1.0 65.5