Hotspot

atpE-trnM-CAU

LSC · 9650-9804 · 155 bp

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Pi 0.119
MarkerSeek score 78.50
Primer available yes
Label atpE-trnM-CAU

Feature Metadata

label_name
atpE-trnM-CAU
feature_type
igs
region
LSC
length_bp
155
variable_sites
18
pi
0.119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE-trnM-CAU_p1 CAGACGAGCTAGAACACGAG GAGTGTTGCTTTCATACGGC 55.0 50.0 57.8 57.5 251-253 bp 1.0 81.4
atpE-trnM-CAU_p2 AGACGAGCTAGAACACGAGT GAGTGTTGCTTTCATACGGC 50.0 50.0 58.2 57.5 250-252 bp 1.0 81.3
atpE-trnM-CAU_p3 TCAGACGAGCTAGAACACGA GAGTGTTGCTTTCATACGGC 50.0 50.0 58.2 57.5 252-254 bp 1.0 81.2
atpE-trnM-CAU_p4 CAGACGAGCTAGAACACGAG GCGGGAGTCATTGGTTCAAA 55.0 50.0 57.8 58.8 233-235 bp 1.0 80.1
atpE-trnM-CAU_p5 AGACGAGCTAGAACACGAGT GCGGGAGTCATTGGTTCAAA 50.0 50.0 58.2 58.8 232-234 bp 1.0 80.0