Hotspot

psbA-trnK-UUU

LSC · 1550-1877 · 328 bp

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Pi 0.0779
MarkerSeek score 80.10
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
328
variable_sites
25
pi
0.0779
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 CAATTACAGAAGCGACCCCA CTAGTTCCGGGTTCGAATCC 50.0 55.0 57.9 57.8 418-516 bp 1.0 82.9
psbA-trnK-UUU_p2 CAATTACAGAAGCGACCCCA CGGCTTGGGGAGAGATTTTT 50.0 50.0 57.9 58.2 490-588 bp 1.0 82.6
psbA-trnK-UUU_p3 ACGGTTTTCAGTGCTGGTTA CTAGTTCCGGGTTCGAATCC 45.0 55.0 57.7 57.8 440-538 bp 1.0 80.8
psbA-trnK-UUU_p4 ACGGTTTTCAGTGCTGGTTA CGGCTTGGGGAGAGATTTTT 45.0 50.0 57.7 58.2 512-610 bp 1.0 80.6
psbA-trnK-UUU_p5 TCCAATTACAGAAGCGACCC CGGCTTGGGGAGAGATTTTT 50.0 50.0 57.6 58.2 492-590 bp 1.0 80.3