Hotspot

trnH-GUG-psbA

LSC · 92-487 · 396 bp

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Pi 0.0799
MarkerSeek score 80.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
396
variable_sites
31
pi
0.0799
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 465-466 bp 1.0 85.1
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 479-480 bp 1.0 84.8
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 478-479 bp 1.0 84.8
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 480-481 bp 1.0 84.8
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCGAGCTAACCTTGGTATGG 55.0 55.0 58.1 57.8 514-515 bp 1.0 83.1