Hotspot

rpl16

LSC · 86227-87729 · 1503 bp

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Pi 0.00133
MarkerSeek score 59.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1503
variable_sites
2
pi
0.00133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AGACCTCTTTCCTTTGGTTCT ACTTTTCCTTATCTTTAACGTTCCA 42.9 32.0 56.5 57.0 1637-1638 bp 1.0 40.1
rpl16_p2 AGACCTCTTTCCTTTGGTTCT ACGTTCCATATTTCGATAAAACA 42.9 30.4 56.5 54.7 1620-1621 bp 1.0 40.1
rpl16_p3 AAGACCTCTTTCCTTTGGTTCT ACTTTTCCTTATCTTTAACGTTCCA 40.9 32.0 57.2 57.0 1638-1639 bp 1.0 40.1
rpl16_p4 AGACCTCTTTCCTTTGGTTCT TCTTTAACGTTCCATATTTCGA 42.9 31.8 56.5 53.6 1626-1627 bp 1.0 40.1
rpl16_p5 AGACCTCTTTCCTTTGGTTCT AACTTTTCCTTATCTTTAACGTTCCA 42.9 30.8 56.5 57.5 1638-1639 bp 1.0 40.1