Hotspot

ccsA

SSC · 117418-118389 · 972 bp

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Pi 0.00103

MarkerSeek score 59.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATGGCATCTTATCTT	AGGGTTCTCGACAATTTATGGG	50.0	45.5	58.8	58.1	1165 bp	1.0	57.1
ccsA_p2	GCGGCATGGCATCTTATCTT	GGGTTCTCGACAATTTATGGGA	50.0	45.5	58.8	58.1	1164 bp	1.0	57.1
ccsA_p3	GCGGCATGGCATCTTATCTT	GGGTTCTCGACAATTTATGGG	50.0	47.6	58.8	56.7	1164 bp	1.0	55.4
ccsA_p4	GCGGCATGGCATCTTATCT	AGGGTTCTCGACAATTTATGGG	52.6	45.5	58.1	58.1	1165 bp	1.0	54.7
ccsA_p5	GCGGCATGGCATCTTATCT	GGGTTCTCGACAATTTATGGGA	52.6	45.5	58.1	58.1	1164 bp	1.0	54.6