Hotspot

atpF

LSC · 51311-52603 · 1293 bp

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Pi 0.00155

MarkerSeek score 47.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1293
variable_sites: 2
pi: 0.00155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TCCATAAGAGGAGATGAGATCA	GCCTATACTCTATACTATAATAGAAGG	40.9	33.3	55.2	53.8	1344 bp	1.0	40.1
atpF_p2	ATCCATAAGAGGAGATGAGATCA	GCCTATACTCTATACTATAATAGAAGG	39.1	33.3	55.8	53.8	1345 bp	1.0	40.1
atpF_p3	TCCATAAGAGGAGATGAGATCAT	GCCTATACTCTATACTATAATAGAAGG	39.1	33.3	55.8	53.8	1344 bp	1.0	40.1
atpF_p4	AGTCTATCCATAAGAGGAGATGAGA	GCCTATACTCTATACTATAATAGAAGG	40.0	33.3	57.7	53.8	1350 bp	1.0	40.1
atpF_p5	CCATAAGAGGAGATGAGATCA	GCCTATACTCTATACTATAATAGAAGG	42.9	33.3	53.6	53.8	1343 bp	1.0	40.1